Plasmid

Part:BBa_K4941039:Design

Designed by: Xinyu Chen   Group: iGEM23_ZJFH-Nanjing   (2023-10-08)


pylxp-CEN1-1-ORI1001-Rep Origin (pMB1)-AmpR-Leu2 marker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3074
    Illegal XhoI site found at 3103
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3960
    Illegal AgeI site found at 2489
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1668
    Illegal BsaI site found at 3245
    Illegal BsaI.rc site found at 3160
    Illegal BsaI.rc site found at 3443
    Illegal SapI.rc site found at 2691


Design Notes

none


Source

yarrowia lipolytica

References